: Utilizing The software package in your qPCR thermocycler, identify at which cycle the fluorescence intensity crosses a user‐specified threshold. Using good and unfavorable controls as benchmarks, choose a threshold that Evidently distinguishes amplification from non‐amplification. ten Commercially offered RNA extraction kits like the Qiagen RNeasy® Mini Plant kit or https://www.pinterest.com/pin/993958580252569364/